Donald F. Doyle Associate Professor Office: 3315 IBB Building Phone: 404-385-0631 Fax: 404-894-2295 E-mail Donald F. Doyle Research Group Web site

B.S., Purdue University; Ph.D., University of North Carolina-Chapel Hill, Research Associate, Howard Hughes Medical Institute-University of Texas Southwestern Medical Center at Dallas

Research Interests

Engineering Nuclear Hormone Receptors Nuclear hormone receptors control the expression of genes in response to small molecule hormones. In performing this activity, the receptors must specifically recognize small molecules, DNA, and other proteins. The amino acids that recognize each of these substrates are varied using genetic engineering techniques until a receptor with novel recognition is created. The original and new receptors are studied using a variety of biophysical techniques to elucidate the principles behind the new activity. This exercise provides both new knowledge for future protein engineering and real materials for research and medical applications.

Representative Publications

"ESPSearch: A program for finding exact sequences and patterns in DNA, RNA, or protein," T. J. Watt and D. F. Doyle, BioTechniques, 2005, 38, 109-115.

"Creation and discovery of ligand-receptor pairs for transcriptional control with small-molecules," L. J. Schwimmer, P. Rohatgi, B. Azizi, K. L. Seley, and D.F. Doyle, Proc Natl Acad Sci USA, 2004, 101, 14707-14712.

"Chemical complementation: small-molecule based genetic selection in yeast," B. Azizi, E. I. Chang, and D. F. Doyle, Biochemical and Biophysical Research Communications, 2003, 303, 774-780.

"Searching for quantitative entropy-enthalpy compensation among protein variants," J. R. Beasley, D. F. Doyle, L. Chen, D. S. Cohen, B. R. Fine, G. J. Pielak, Proteins: Structure, Function, and Genetics, 2002, 49, 398-402.

"Gas phase activity of anti-FITC antibodies on a surface acoustic wave resonator device," D. D. Stubbs, W. D. Hunt, S. H. Lee, D. F. Doyle, Biosen. Bioelectron., 2002, 17, 471-477.

"Inhibition of gene expression inside cells by peptide nucleic acids: effect of mRNA target sequence, mismatched bases, and PNA length," D. F. Doyle, D. A. Braasch, C. S. Simmons, B. A. Janowski, D. R. Corey, Biochem., 2001, 40, 53-64.

"Engineering orthogonal ligand-receptor pairs from "near drugs", D. F. Doyle, D. A. Braasch, L. K. Jackson, H. A. Weiss, M. F. Boehm, D. J. Mangelsdorf, D. R. Corey, J. Am. Chem. Soc., 2001, 123, 11,367-11,371.

"Modifying ligand specificity of gene regulatory proteins," D. F. Doyle, D. J. Mangelsdorf, D. R. Corey, Curr. Opin. Chem. Biol. 2000, 4, 60-63.

"Engineering proteins: keeping up with biology," D. F. Doyle, D. R. Corey, Chem. Biol., 1998, 5, R157-R160.

"Engineering novel specificities for ligand-activated transcription in the nuclear hormone receptor RXR," D. J. Peet, D. F. Doyle, D. R. Corey, D. J. Mangelsdorf, Chem. Biol., 1998, 5, 13-21.