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Stephen HarveyProfessor and Eminent Scholar in Biology Office: Cherry Emerson Phone: 404-894-3700 Fax: 404-894-0519 |
Ph.D., Biophysics, Dartmouth College, 1971
Research Interests
Professor Harvey's research group is interested in macromolecular structure and dynamics and the relationship of these to biological function.
Structure-function relationships in the ribosome. The structures of the ribosomal large and small subunits have recently been determined by X-ray crystallography. Cryo-electron microscopic studies of complexes of mRNA, tRNA, and various cofactors have captured the ribosome at several points in the translational cycle, but only at low resolution. Dr. Harvey and collaborators are combining the data from these and other experiments into detailed models with the aim of determining the structural, thermodynamic, and kinetic basis of translational initiation, fidelity, elongation and termination. He is also interested in the evolution of ribosome structure, and in ribosome assembly.
Lipoproteins and atherosclerosis. The group is collaborating with several experimentalists to incorporate all available information into detailed atomic models for high and low density lipoproteins (HDLs and LDLs), in an effort to understand structure-function relationships in these particles.
Viral assembly. Understanding the mechanisms whereby viruses package their nucleic acids should offer new opportunities for drug design. Collaborations are aimed at understanding the structural and dynamic aspects of assembly of both DNA viruses and RNA viruses.
Selected Publications
“Assembly of Lipoprotein Particles Containing Apolipoprotein-B: Structural Model for the Nascent Lipoprotein Particle,” P. E. Richardson, M. Manchekar, N. Dashti, M. K. Jones, A. Beigneux, S. G. Young, S. C. Harvey and J. P. Segrest, Biophys. J., 2005, 88, 2789-2800.
“Packaging Double-Helical DNA into Viral Capsids,” J. C. LaMarque, T. L. Le and S. C. Harvey, Biopolymers, 2004, 73, 348-355.
“A Structural Model for the Assembly of the 30S Subunit of the Ribosome," S. M. Stagg, J. A. Mears and S. C. Harvey, J. Mol. Biol., 2003, 328, 49-61.
"Study of the Structural Dynamics of the E. coli 70S Ribosome Using Real Space Refinement,” H. Gao, J. Sengupta, M. Valle, A. Korostelev, N. Eswar, S. M. Stagg, P. VanRoey, S. C. Harvey, R. K. Agrawal, A. Sali, M. S. Chapman and J. Frank, Cell, 2003, 113, 789-801.
"Building a Minimal Ribosome Based on Comparative Sequence Analysis," J. A. Mears, J. J. Cannone, S. M. Stagg, R. R. Gutell, R. K. Agrawal and S. C. Harvey, J. Mol. Biol., 2002, 321:215-234.
"Investigation of Viral DNA Packaging Using Molecular Mechanics Models," J. Arsuaga, R. K. Z. Tan, M. Vazquez, D. W. Sumners and S. C. Harvey, Biophys. Chem., 2002, 101-102:475-484.
"Molecular Dynamics Simulations on Discoidal HDL Particles Suggest a Mechanism for Rotation in the Apo A-I Belt Model," A. E. Klon, J.P. Segrest and S.C. Harvey, J. Mol. Biol., 2002, 324:703-721.
"Motifs in Nucleic Acids: Molecular Mechanics Restraints for Base Pairing and Base Stacking," S. C. Harvey, C. Wang, S. Teletchea and R. Lavery, J. Comput. Chem., 2003, 24:1-9.
"Structural Studies of the tRNA Domain of tmRNA," S. M. Stagg, A. A. Frazer-Abel, P. J. Hagerman and S. C. Harvey, J. Mol. Biol., 2001, 309:727-735.
"Molecular Belt Models for the Apolipoprotein A-I Paris and Milano Mutations," A. E. Klon, M. K. Jones, J. P. Segrest and S. C. Harvey, Biophys. J., 2000, 79:1679-1685.