- Vasser Woolley Chair
- Senior Editor, Journal of Physical Chemistry
- Outstanding Achievement in Research Program Development Award
- Visiting Lecturer, National Science Council, Taiwan
- Camille Dreyfus Teacher-Scholar Award
- Blanchard Assistant Professor
- Alfred P. Sloan Foundation Fellow
- National Science Foundation, Faculty Early CAREER Award
Dr. Dickson's group is developing novel spectroscopic and imaging methods for the study of intermolecular interactions and dynamics in biological systems.
New florophores for biological imaging. Standard fluorescent labels are often unsuitable for long-time single molecule imaging, especially in living systems with high background emission. Thus, in order to improve contrast, we are developing Au and Ag nanoclusters as a new class of fluorescent labels in biology. These high brightness, robust nanomaterials should enable direct labeling of proteins to image live cells, study protein-protein interactions, and potentially watch individual proteins as they fold to their native conformations. Au and Ag nanoclusters exhibit discrete excitation and emission due to being composed of only a few atoms. Consequently, with size-tunable optical properties and absoprtion comparable to semiconductor quantum dots, but with improved photostability, these nanoclusters offer new opportunities in biological labeling. For example, the extremely small size will be less invasive; noble metals are not toxic; and their discrete energy levels enable energy transfer experiments to be performed—all with weak mercury lamp illumination on the single molecule level. Much brighter and more robust than organic dye molecules, these advanced inorganic nano-materials are being utilized both as optical memory elements and as photo-activated biological labels.
Optical Properties of Individual Nanoparticles. We have created and are currently studying the properties of extremely fluorescing Ag clusters . Having made these water-soluble, the outstanding photostability and brightness of DNA-encapsulated Ag nanocluster emitters offer great promise as materials and biological labels.
SAFIRe. We have developed the concept of optically modulated fluorescence for selective recovery of our emitters from within overwhelming background. This adapts the concept of modulation as used in traditional high resolution absorption spectroscopy to fluorescence imaging, and has allowed discrimination of nanocluster, cyanine dye, and fluorescent protein signals with signal:background improvements exceeding 100-fold. This expands the dimensionality of imaging to include dark state lifetimes, and is performed by modulating long-wavelength transient absorptions of our designed emitters. Such methods do not increase background, but simply shift our signals of interest to a unique detection frequency, enabling its selective recovery without background. Interaction timescales and selective imaging enable a wide variety of protein interactions and locations to be studied.
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Senior Editor – The Journal of Physical Chemistry B