Dr. Caitlin Davis

MoSE 3201A
Tuesday, January 22, 2019 - 4:00pm to 5:00pm

Protein dynamics: Connecting in vitro, in cell, and in vivo

Although biomolecules evolved to function in the cell, most biochemical and biophysical studies have been carried out in vitro. A combination of in vitro, in-cell, and in vivo studies will highlight how steric and non-steric interactions modulate protein folding and protein-RNA interactions. I will introduce a customized pipeline that combines meganuclease mediated transformation with fluorescence-detected temperature-jump microscopy to image fast dynamics of biomolecules in living zebrafish with single-cell resolution. To interpret in vivo and in-cell results, an in vitro systematic series of solvation environments will distinguish contributions from non-steric and steric interactions to stability, compactness, and kinetics. Our results demonstrate that different cellular environments of different tissues contribute to different protein stability and kinetic phenotypes.

Contact Information: 
Host:  Loren Williams
Map of Georgia Tech

School of Chemistry & Biochemistry

901 Atlantic Drive Atlanta, GA 30332-0400

(404) 894-4002 (phone) | (404) 894-7452 (fax)