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Marchello Cavitt awarded the California Alliance for Graduate Education Postdoctoral (AGEP) Fellowship

Marchello Cavitt awarded the California Alliance for Graduate Education Postdoctoral (AGEP) Fellowship

Evelyn Maris and Breanne Hamlett are awarded ASEE SMART Fellowships

Evelyn Maris and Breanne Hamlett have each received the 2015 Science, Mathematics & Research for Transformation (SMART) Fellowship from the American Society for Engineering Education (ASEE). The Fellowship will support their dissertation studies and provide professional summer internships and post-doctoral work at the Defense Forensic Science Center (DFSC) in Forest Park, GA.

Prof. Rigoberto Hernandez Appointed as Phi Beta Kappa Society Visiting Scholar

Each year, top scholars in the liberal arts and sciences are selected to interact with and mentor Phi Beta Kappa students throughout the U.S.

Stefan France and Wendy Kelly Recognized for Mentorship

Two School of Chemistry and Biochemistry professors guide students and colleagues alike in science and career development.

Seth Marder Honored by the Materials Research Society

Regents’ Professor of Chemistry Seth Marder is the straightforward choice for a singular honor, for nonlinear reasons…

Reynolds group featured on ACS Headline Science

Professor John Reynolds and group publish new research discussing their “sunglasses on demand”, a polymer coating developed with the push of a button changes color! This research has been highlighted in the latest episode of ACS Headline Science.

Charlie Liotta: The Man, The Myth, The Legend

On February 26th people from across the country gathered at Georgia Tech to honor Charlie Liotta, Regents Professor Emeritus in the School of Chemistry and Biochemistry, and his 50 years of teaching and research.

Wu wins NSF CAREER Award

School of Chemistry and Biochemistry Assistant Professor Ronghu Wu has been awarded the National Science Foundation’s most prestigious award for early-career faculty.

Colorful and Dynamic Workshops

School of Chemistry and Biochemistry Professor Rigoberto Hernandez and Research Scientist Kyril Solntsev are organizing unique workshops in the mountains.

Seminars & Events

Meeting - Thursday, June 11, 2015 - 11:00am - MoSE 2100F
Meeting - Tuesday, June 16, 2015 - 11:00am - MoSE 3201A
Meeting - Thursday, July 16, 2015 - 11:00am - MoSE 2100F
Meeting - Tuesday, July 21, 2015 - 11:00am - MoSE 3201A

Featured Research

Article Title
Research Authors
Johanna M. Smeekens, Weixuan Chen, Ronghu Wu .
Citation
Journal of the American Society for Mass Spectrometry (2015), Vol. 26(4), 604-614
Miscellaneous Details
This work was supported by start-up funds from Georgia Institute of Technology. R.W. is also supported by the Blanchard Assistant Professorship.

Cell surface N-glycoproteins play extraordinarily important roles in cell-cell communication, cell-matrix interactions, and cellular response to environmental cues. Global analysis is exceptionally challenging because many N-glycoproteins are present at low abundances and   effective separation is difficult to achieve. Here, we have developed a novel strategy integrating metabolic labeling, copper-free click chemistry, and mass spectrometry (MS)-based proteomics methods to analyze cell surface N-glycoproteins comprehensively and site-specifically. A sugar analog containing an azido group, N-azidoacetylgalactosamine, was fed to cells to label glycoproteins. Glycoproteins with the functional group on the cell surface were then bound to dibenzocyclooctyne-sulfo-biotin via copper-free click chemistry under physiological conditions. After protein extraction and digestion, glycopeptides with the biotin tag were enriched by NeutrAvidin conjugated beads. Enriched glycopeptides were deglycosylated with peptide-N-glycosidase F in heavy-oxygen water, and in the process of glycan removal, asparagine was converted to aspartic acid and tagged with 18O for MS analysis. With this strategy, 144 unique N-glycopeptides containing 152 N-glycosylation sites were identified in 110 proteins in HEK293T cells. As expected, 95% of identified glycoproteins were membrane proteins, which were highly enriched. Many sites were located on important receptors, transporters, and cluster of differentiation proteins. The experimental results demonstrated that the current method is very effective for the comprehensive and site-specific identification of the cell surface N-glycoproteome and can be
extensively applied to other cell surface protein studies.

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