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Heme, a Poisonous Nutrient, Tracked by ‘Green Lantern’ Sensor

Reddi lab features in Research Horizons for recent PNAS paper

Meet Casey Bethel, Georgia's 2017 Teacher of the Year

College of Sciences basks in the reflected glow of a GIFTed science teacher

New award recognizes Wendy Kelly, Joseph Sadghi, and William Baron

Congratulations to Professors Wendy Kelly, Joseph Sadighi and William Baron as the inaugural recipients of the Vasser Woolley Award for Excellence in Instruction in the School of Chemistry and Biochemistry.

Kristy S. Syhapanha Puts a Foot in the Door of Her Dream Career

Kristy Southysa Syhapanha never thought of studying at Georgia Tech. She didn’t think she would qualify, so she didn’t bother – at first.

Missing Links Brewed in Primordial Puddles?

Researchers have achieved a significant advancement toward unlocking a longstanding evolutionary mystery -- how components of RNA and DNA formed from chemicals present on early Earth before life existed.

Welcome to Dr. Gutekunst

The School of Chemistry and Biochemistry is pleased to welcome Dr. Will R. Gutekunst, who will join the faculty on July 1, 2016 as an Assistant Professor.

Welcome to Dr. La Pierre

The School of Chemistry and Biochemistry is pleased to welcome Dr. Henry S. La Pierre, who will join the faculty on July 1, 2016 as an Assistant Professor with a courtesy appointment in the George W. Woodruff School of Mechanical Engineering and the Nuclear and Radiological Engineering and Medical Physics Program.

Seminars & Events

Meeting - Tuesday, August 16, 2016 - 11:00am - MoSE 3201A
Prof. Kenneth Brown - Georgia Institute of Technology
Colloquium - Thursday, September 1, 2016 - 4:00pm - MoSE G011
Prof. Christine Payne - Georgia Institute of Technology
Colloquium - Thursday, September 8, 2016 - 4:00pm - MoSE G011
Meeting - Tuesday, September 20, 2016 - 11:00am - MoSE 3201A

Featured Research

Article Title
Research Authors
Kodlekere, Purva; Cartelle, Anabel Liyen; Lyon, L. Andrew.
Citation
RSC Advances (2016), Vol. 6(38), 31619-31631
Miscellaneous Details
Prof. Thomas Barker for access to tissue culture facilities at Georgia Tech and Dr Ashley Carson Brown for providing training. Prof. Robert Dickson and Aida Demissie. Dr Nicole Welsch.

We present the development and detailed characterization of a range of amine functionalized microgels for utilization in conjugation reactions. Cationic N-isopropylmethacrylamide (NIPMAm) based microgels were synthesized through copolymerization with a primary amine containing monomer, N-(3-aminopropyl) methacrylamide hydrochloride (APMA). A range of synthesis conditions and monomer feed ratios generated microgels of diverse architectures, in different size ranges and with varying amounts of incorporated primary amines. The efficiency of amine incorporation was quantified using a fluorescence-based assay in order to determine the potential applicability of these particles for controlled bioconjugation reactions. The pH responsivity of all microgels was studied via dynamic light scattering and their height profile was investigated through atomic force microscopy following deposition on a functionalized flat substrate. Tunable resistive pulse sensing was employed to characterize microgels with respect to their number densities and molecular weights. These microgels were then conjugated to two dyes, malachite green and rose bengal with the purpose of investigating accessibility of primary amine groups for conjugation reactions. The microgel-dye constructs were then analyzed for dye content/ microgel. Finally, the viability of NIH 3T3 fibroblasts incubated in the presence of varying concentrations of non-conjugated and dye conjugated microgels was studied. Confocal imaging revealed low cellular toxicity under conditions of incubation with low concentrations of microgel-dye conjugates, which is promising for eventual utilization of these constructs in bioimaging applications.

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